电离辐射通过转化生长因子-β-介导的结缔组织-间质转换来促进癌细胞的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

摘要 ：探讨强磁场是不是可通过转成介素-β（TGF-β）-激活的角质层-粘液转成 (EMT)来促进肝肝细胞的波及移往。使用工业产值2Gy(60)Coγ线光线由来人类肝脏的6种肝肝细胞，记录下来与EMT无关的变化，这包括分别运用显微镜新技术，抗原质；还有方法，免疫荧光新技术，穿孔实验和Transwell小室实验来捕捉到并检测肝细胞组织有机体，EMT标记，波及移往能力等。采用酶嗣后免疫吸附法检测这些肝肝细胞中TGF-β抗原水准，运用特别抑止剂SB431542来评估TGF-β频谱通路在强磁场EMT中的作用。经过工业产值为2Gy光线的肝肝细胞中存有间叶肝细胞的表达，与假光线组相较其角质层标记减小，间叶肝细胞标记增加，同时其波及转回能力提升，TGF-β抗原水准也提升。实质性见到由A549强磁场诱导的EMT可通过对TGF-β频谱抑止发生逆转。这些分析表明TGF-β激活的EMT在强磁场诱导提升肝肝细胞波及转回能力中起着极为重要。

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